Anti-microbial resistance (AMR) is a global emergence of drug resistant strains, leading to a wide variety of infections in humans. SrtA plays a pivotal role in the pathogenic processes of bacterial infection, thus SrtA is considered as a promising target for antimicrobial agents. A library of antimicrobial peptides has been assessed for synergistic activity in combination with a library of naturally derived sortase A inhibitors to inhibit important bacterial enzyme called sortase A enzyme SrtA). Checkerboard technique was used, that appears useful for determining antimicrobial synergism against Methicillin-resistant Staphylococcus aureus (MRSA) and synergistic combinations were identified. The synergistic combinations allowed for up to 4-fold reduction in the concentration of the antimicrobial peptide, which is expected to reduce any associated toxicity and costs associated with their use. Inhibition of bacterial growth has been readout.